Dose-Dependent Changes in the Levels of Oxidative Stress Biomarkers in the Muscle Tissue of Rainbow Trout (Oncorhynchus mykiss Walbaum) after In Vitro Treatment by Extracts Derived from Stalks and Roots of Great Celadine (Chelidonium majus L.)

Consistent with our previous studies, we continue to evaluate the antioxidant potential of representatives belonging Papaveraceae family collected from northern part Poland using a muscle tissue model rainbow trout (Oncorhynchus mykiss Walbaum). Therefore, in present study, oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), protein modification carbonyl derivative content, total capacity (TAC)] were used activity stalk and root extracts Chelidonium majus L. (CM) at doses 5 mg·mL-1, 2.5 1.25 0.63 mg·mL-1. Muscle homogenates this study. Phosphate buffer was as positive control. After incubation mixture 25 °C for 2 h continuous mixing, samples biochemical studies. Results study revealed that dose CM mg·mL-1 showed highest trout. The derived mainly roots rural areas effective reducing levels by lipid peroxidation markers, which may suggest active such alkaloids (chelidonine, sanguinarine, berberine), flavonoids, phenols these plants can effectively protect membrane structures cells salmonids. We also observed statistically significant reductions both aldehydic ketonic derivatives oxidatively modified proteins after compared controls. comparison results shows inhibit damage scavenging free radicals and/or activation defenses. secondary metabolites CM, i.e. polyphenols alkaloids, are most likely responsible effect. Using vitro increases TBARS OMP observed. Screening species other biological activities, including activity, is essential be search preventive measures pathogenesis diseases caused human veterinary medicine.